ABSTRACT
Interferon regulatory factor 7 is originally identified in the context of Epstein-Barr virus infection,and has play a key role in regulator of type Ⅰ interferons.Aberrant production of type Ⅰ interferons is associated with many types of diseases such as cancers and autoimmune disorders.Furthermore,mounting evidence has shed light on the importance of interferon regulatory factor 7,which is a multifuwtional transcription factor,not only regulate the response of inflammation and immune in body,but also regulate others biological funetions such as tumor formation,cell invasion and so on.The article has reviewed the progression of both interferon regulatory factor 7 and tumor.
ABSTRACT
Objective To investigate the effects of Krüppel like factor 4 (KLF4)on matrix metalloproteinase 9 (MMP9)ex-pression in hepatocellular carcinoma(HCC).Methods A total of 50 primary hepatocellular carcinoma samples and their correspond-ing adjacent tissues specimens were collected.The expression of KLF4 and MMP9 were detected by IHC,Western blot and qRT-PCR.After KLF4 gene was transfected into hepatocellular carcinoma cell line (HepG2 cell line),the expressions of KLF4 and MMP9 were conformed by qRT-PCR and Western blot.Migration and invasion of HepG2 cell line transfected by KLF4 were detec-ted by wound-healing assay and invasion assay.Results Compared to corresponding adjacent tissues,The expression of KLF4 was significantly lower in HCCs(P <0.05),and MMP9 expression was remarkably higher in HCCs(P <0.05).KLF4 over-expression inhibited the expression of MMP9 on the protein and mRNA levels.Wound-healing assay and invasion assay confirmed that KLF4 regulated cell invasion and migration through regulating MMP9 expression.Conclusion KLF4 showed low expression in HCCs,and MMP9 was overexpressed.Up-regulation of KLF4 could decrease the expression of MMP9 in HepG2 cell line,which inhibited inva-sion and migration.